Research in the Winkel Lab focuses on understanding the intracellular organization of metabolism, with the Arabidopsis flavonoid pathway as the experimental model. Twenty five years of work has revealed a surprising assembly of the core enzymes around chalcone synthase, the entry point into the pathway, and evidence that flux into terminal branches is controlled by competitive enzyme interactions. Moreover, although the core enzymes are primarily known for their role in flavonoid biosynthesis in the cytoplasm, we have found that chalcone synthase may also participate in epigenetic control of gene expression in the nucleus.
Our group has a new EAGER project funded by the NSF Systems and Synthetic Biology Program, a collaboration with Deb Kelly‘s Lab at Penn State and the Helm Lab/MSI at Virginia Tech. The project will develop novel cyro-EM-based and advanced proteomics technologies to visualize and characterize large dynamic complexes in plants.
We have recently completed an NSF Arabidopsis 2010-funded project with the Helm Lab/MSI and with the Muday and Fetrow groups at Wake Forest to develop a systems-level understanding of the auxin and ethylene response in seedling roots.
The Winkel Lab maintained a 22 year-long partnership with the Brewer group in Virginia Tech’s Department of Chemistry on developing novel multimetallic anti-cancer agents. This work was recognized with a Breakthrough Award from Popular Mechanics in 2010. Karen Brewer was lost to us in 2014, but her legacy is carried forward by her many students, colleagues, and friends.
Supported by the National Science Foundation for 25 years